Tuesday 15 May 2018

bacteriology




characters include the characters and property of cells, behaviors
of the biological system. The phenotypic assay is accomplished in 96 well Petri
plates.the study of phenotypic characters helps one to study the cell
transformation, microscopic and macroscopic morphology. Using the phenotypic
characters bacterial identification and microbial ecology are done.microbial
metabolomics is integrated component of biology. Recent advancements in
metabolomics are the analysis of metabolome. The phenotypic classification system
of gram
stain bacteria includes both gram-negative and gram-positive bacteria.some
factors influencing phenotypic characters include changing environment,
radiation, toxins, mutagens, bacteriophages etc. Some
phenotypic changes include antigenic
variations, competence in Bacillus subtilis, endospore in Bacillus subtilis,
colicin production in E.coli, the persistence of antibiotic-resistant
Staphylococcus and phenotypes due to mutation.Genotypic
characters have application in bacterial identification and classification by
genotypic methods which involve genetic materials DNA and RNA. Species-specific
hybridization is a technique which involves DNA-DNA hybridization
and thermal stability hybrids are used in differentiating species.some of the
genotypic methods include amplified ribosomal DNA restriction analysis,16s
and 23s rDNA sequencing, strain-specific typing, pulse field gel electrophoresis,
randomly amplified polymorphic DNA assay,amplified fragment length
polymorphism, ribotyping, Denaturing gradient gel electrophoresis and finally
protein-based methods.

To know more visit https://bacteriology.pulsusconference.com/


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